Simultaneous nanostructure and chemical imaging of intact whole nematodes

MWM Jones; NW Phillips; B Abbey; DJ Hare; GA Van Riessen; DJ Vine; MD De Jonge; G McColl

Journal article

Simultaneous nanostructure and chemical imaging of intact whole nematodes

MWM Jones, NW Phillips, B Abbey, DJ Hare, GA Van Riessen, DJ Vine, MD De Jonge, G McColl

Chemical Communications | ROYAL SOC CHEMISTRY | Published : 2019

DOI: 10.1039/c8cc09664c

Abstract

Biological X-ray fluorescence microscopy (XFM) is an important tool for determining quantitative distributions of bioinorganics and essential trace elements. Here we present a new analysis approach for rapid nanoscale ptychographic imaging and simultaneous chemical mapping of large radiation sensitive specimens without image degradation associated with probe evolution.

University of Melbourne Researchers

Dominic Hare Author

Gawain McColl Author

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Awarded by National Institutes of Health

Funding Acknowledgements

We thank Dectris Ltd, Baden, Switzerland, for loan of the EIGER X 1M detector. This research was undertaken on the XFM beamline at the Australian Synchrotron, part of ANSTO, Victoria, Australia, and supported by the Multi-modal Australian ScienceS Imaging and Visualization Environment (MASSIVE) (http://www.massive.org.au) and the Victorian Government's Operational Infrastructure Support Program. The authors acknowledge the support of the Caenorhabditis Genetics Center of the US National Institutes of Health - Office of Research Infrastructure Programs (P40OD010440) for materials. The Australian Research Council (ARC) Centre of Excellence for Advanced Molecular Imaging (CE140100011), and DP130100357 and DP180101248 and National Health and Medical Research Council Career Development Fellowship (GNT1122981; supported by Agilent Technologies) schemes funded this work. DJH receives research and material support from Agilent Technologies.